DNA damage is a proven biomarker of radiation effects. Commonly used cytogenetic techniques to detect DNA strand breaks include chromosomal aberrations, micronucleus and comet testing in the field and laboratory. In recent times, flow cytometry (FCM) has been widely used to quantify DNA damage because it allows analyzing high numbers of nuclei within minutes, providing statistically reliable results in a short period of time. Ideally, all cells in an organism contain the same amount of DNA. DNA damage results from the breakage and rearrangement of chromosomes and interference with normal chromosome segregation during cell division. Double-strand breaks are the most significant DNA lesions caused by ionizing radiation and other damage from exposure to genotoxic agents, resulting in cells with abnormally high or low DNA content . This variability can be detected by an increase in the coefficient of variation (CV) of cells in G0/G1 phase, measured by flow cytometry. Alternatively, appearance of aneuploid cell populations following mitotic machi....