Fresh mushrooms have been thoroughly washed in clean water, cut into pieces and air dried. The parts of the edible mushrooms such as spores, stem and cap were placed in the center of an agar plate containing the optimized medium. The Petri dishes containing the cultures were incubated at 30°C for 7 days. Mycelial growth was assessed by mycelial diameter for 7 days. All experiments mentioned in this article were carried out