Methods for production of alkaline protease from Bacillus Cereus strain Bacillus cereus strain S8 (MTCC NO 11901) was made using statistically optimized media supplemented with molasses, 1% (w/v); potassium nitrate, 0.75% (w/v); saline solution, 5% (v/v); {MgSO4.7H2O, 0.5% (w/v); KH2PO4, 0.5% (w/v)}; FeSO4.7H2O, 0.01% (w/v) and CaCO3, 0.5% formulated by [3].Say no to plagiarism. Get a tailor-made essay on “Why violent video games should not be banned”? Get the original essay Deproteinization of Shrimp Waste The shrimp waste (50%, w/v) was chopped and cooked at 100°C for 20 minutes to inactivate endogenous enzymes. The cooked sample was homogenized for 2 minutes in a Moulinex® blender. Adjust the pH of the mixture to 10.0, and then the shrimp waste proteins were digested with crude protease (208 ± 0.89 U/ml). The reaction was stopped after 3 hours of incubation at 75°C by heating the solution for 20 minutes at 100°C. Then, the shrimp waste protein hydrolyzate was centrifuged for 20 min at 5000 g to separate the soluble and insoluble fractions. Wash the solid phase with distilled water and dry for 1 hour at 60°C. Deproteinization (DP) was expressed as a percentage and calculated by the following equation described by Rao et al., (2000).% DP = [(PO Г— O) - (PR Г— R)] Г— 100PO Г — Owhere PO and PR are the protein concentrations (%) before and after hydrolysis; while O and R represent respectively the mass (g) of the original sample and the hydrolyzed residue in dry weight. Silk degumming method Raw silk yarns were dried at 80 °C to constant weight and then treated with protease from Bacillus cereus strain S8 at 70 °C in Glycine-NaOH buffer (pH 10.0) for 1 h. Wash the treated fibers with water and dry them at 80°C. The difference in weight between treated and untreated silk threads was measured. The structures of the treated and untreated fibers were observed using a scanning electron microscope. Determination of weight loss The weight loss of the fabric was recorded as the weight loss of the dried sample. Drying conditions were 80 °C in a circulating air oven for 1 h. The samples were weighed after cooling in a desiccator. The following equation was used to calculate the weight loss (% by weight): % by weight =("W1-W2" /"W1" )"x100"Where W1 and W2 are the weights of the fabric before and after respectively treatment (Kalantzi et al., 2008). Hair Removal Studies Hair removal of goat skins preserved in common salt purchased from the local market was cut into two pieces. One part was chosen to be dehaired using lime sulphide to serve as a control in the study and the other was taken for enzyme application trials. The hides were soaked with three changes of water until free of dirt, feces, blood and other contaminating materials and also free of sodium chloride, as verified by nitrate solution money. After soaking, the skins were stacked to drain water for about an hour before application. The control was applied evenly on the flesh side with a paste of lime (10%) and 3% sodium sulphide. Another piece was immersed in 50 ml of Glycine-NaOH buffer (pH 10.0) supplemented with protease (206 ± 0.19 U/ml). After application, they stayed overnight. The next day (after approximately 6 p.m.), the skins were manually dehaired on a wooden beam using a knife consistent with commercial practices in the leather industry..

Essay / Methods for production of alkaline protease from Bacillus Cereus strain Bacillus cereus strain S8 (MTCC NO 11901) was made using statistically optimized media supplemented with molasses, 1% (w/v); potassium nitrate, 0.75% (w/v); saline solution, 5% (v/v); {MgSO4.7H2O, 0.5% (w/v); KH2PO4, 0.5% (w/v)}; FeSO4.7H2O, 0.01% (w/v) and CaCO3, 0.5% formulated by [3].Say no to plagiarism. Get a tailor-made essay on “Why violent video games should not be banned”? Get the original essay Deproteinization of Shrimp Waste The shrimp waste (50%, w/v) was chopped and cooked at 100°C for 20 minutes to inactivate endogenous enzymes. The cooked sample was homogenized for 2 minutes in a Moulinex® blender. Adjust the pH of the mixture to 10.0, and then the shrimp waste proteins were digested with crude protease (208 ± 0.89 U/ml). The reaction was stopped after 3 hours of incubation at 75°C by heating the solution for 20 minutes at 100°C. Then, the shrimp waste protein hydrolyzate was centrifuged for 20 min at 5000 g to separate the soluble and insoluble fractions. Wash the solid phase with distilled water and dry for 1 hour at 60°C. Deproteinization (DP) was expressed as a percentage and calculated by the following equation described by Rao et al., (2000).% DP = [(PO Г— O) - (PR Г— R)] Г— 100PO Г — Owhere PO and PR are the protein concentrations (%) before and after hydrolysis; while O and R represent respectively the mass (g) of the original sample and the hydrolyzed residue in dry weight. Silk degumming method Raw silk yarns were dried at 80 °C to constant weight and then treated with protease from Bacillus cereus strain S8 at 70 °C in Glycine-NaOH buffer (pH 10.0) for 1 h. Wash the treated fibers with water and dry them at 80°C. The difference in weight between treated and untreated silk threads was measured. The structures of the treated and untreated fibers were observed using a scanning electron microscope. Determination of weight loss The weight loss of the fabric was recorded as the weight loss of the dried sample. Drying conditions were 80 °C in a circulating air oven for 1 h. The samples were weighed after cooling in a desiccator. The following equation was used to calculate the weight loss (% by weight): % by weight =("W1-W2" /"W1" )"x100"Where W1 and W2 are the weights of the fabric before and after respectively treatment (Kalantzi et al., 2008). Hair Removal Studies Hair removal of goat skins preserved in common salt purchased from the local market was cut into two pieces. One part was chosen to be dehaired using lime sulphide to serve as a control in the study and the other was taken for enzyme application trials. The hides were soaked with three changes of water until free of dirt, feces, blood and other contaminating materials and also free of sodium chloride, as verified by nitrate solution money. After soaking, the skins were stacked to drain water for about an hour before application. The control was applied evenly on the flesh side with a paste of lime (10%) and 3% sodium sulphide. Another piece was immersed in 50 ml of Glycine-NaOH buffer (pH 10.0) supplemented with protease (206 ± 0.19 U/ml). After application, they stayed overnight. The next day (after approximately 6 p.m.), the skins were manually dehaired on a wooden beam using a knife consistent with commercial practices in the leather industry..

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