Tissue culture has been practiced since the early 1900s. Since its beginnings, many advances have been made in producing a viable plant from the culture. Tissue culture is defined as the growth of plants from plant tissues in an artificial medium and sterile environment. Uses of this technique include food processing, agriculture, pharmaceuticals and medicines. This has an influence on human well-being, such as food processing, human health and environmental protection. There is a growing demand for tissue culture, which is why it is becoming more and more popular to further explore its commercial potential. Tissue culture is also used for the production of pathogen-free plants, preservation of genetic material, and propagation throughout the year. Callus Culture – Callus formation is a mass of undifferentiated cells from an explant. Auxins and cytokinins are necessary for proper development. Transplanting should be done every 3 to 5 weeks. This type of culture is mainly intended for the maintenance of cell lines or morphogenesis. Suspension Culture – Cells are cultured in a liquid medium to form a callus. Subculture should be done every 3 to 14 days due to their faster growth rate than callus culture. This is the most widely used method in large-scale production. Single cell culture – Single cell culture produces a clone of identical cells under in vitro conditions. Pieces of leaves are macerated in a mortar and pestle with a pad, then spun in a centrifuge. The cells are then cultured on a feed medium. Micropropagation – This method uses a mature cell to dedifferentiate into callus tissue. The two processes of micropropagation are known as organogenesis and somatic embryogenesis. Organogenesis develops shoot buds into apical meristems, while somatic embryogenesis forms non z...... middle of paper ......then disinfected in 10% bleach solution for 15 minutes. It is important to put all equipment needed for cultivation in the laminar flow hood to prevent the spread of contaminants once you start working. After thoroughly washing your hands and arms, remove the leaf from the bleach solution with sterile forceps, then sterilize the forceps and scalpel again before cutting the petiole into 1 cm pieces and the leaf into squares of approximately 2 cm. Place the explants on a Petri dish with media, pressing gently to ensure contact between the explant and the media. The explants are first placed on an initiation medium, then then placed on a development medium. The medium used depends on the stage of growth and the needs for salts, sugars, nutrients, vitamins, hormones and pH. This process ultimately produces numerous seedlings that can be transplanted into a non-sterile environment..