Table of contentsWriting from the laboratory ADiscussion: Writing from the BLR laboratoryEditing from the C laboratoryConclusionLab Writing a diffusion: Say no to plagiarism. Get a tailor-made essay on “Why Violent Video Games Should Not Be Banned”? Get the original essay Objective/Question: In a plasma membrane where the net movement of molecules changes from a higher concentration to a lower concentration, will the dialysis bag turn blue because of the diffusion of glucose and of IKI in the dialysis bag? Hypothesis: If I added the glucose and IKI to the distilled water, I hypothesize that the water with the IKI solution will diffuse into the dialysis bag and will take on the blue color.Objective: The objective of the laboratory is to see and understand the semi-permeable membrane and how the diffusion of molecules from high concentration to low concentration works. The goal is to add the IKI into the water and observe whether or not the IKI will diffuse into the glucose/starch solution. Equipment: Dialysis tubing, plastic cup, glucose/starch solution, distilled water, iodine-potassium iodide (IKI) solution. , drip pipette, glucose test strips, beaker.Procedure:Test two locations: the dialysis bag and the cup. Test two solutions: IKI and glucose/starch solution. For data collection, construct a table and in the table show/describe the color of the initial solution and the color of the final solution for each. Also report the blood glucose test results, using the + symbol for a positive test result and the - symbol for a negative test result. Pour 160 to 170 ml of distilled water into a plastic cup. Next, perform a Benedict test for the monosaccharide and record the initial glucose test results in a table. Then add approximately 4 ml of IKI solution to the water, mix well and record the initial color of the solution in Table 1. Perform Benedict's test for monosaccharide for glucose/starch solutions and record the data. Obtain a piece of dialysis tubing that has been soaked in water. Since the tube is soft and bendable, roll it between your thumb and index finger to open it. With string, tie one end of the bag to form a bag. Using a small funnel, pour 15 mL of glucose/starch solution into the dialysis bag and smooth the top of the bag by passing it between your thumb and index finger to keep out air. Tie off the top end of the bag, but leave plenty of room in the bag for expansion. Record the initial color of the glucose/starch solution in Table 1. Dip the dialysis bag into the solution in the cup and ensure that the part of the bag containing the glucose/starch solution is completely covered by the solution in the cup at any time. .While waiting 30 minutes, perform the following exercise. Create a drawing of your experiment (dialysis bag in the dish) and call it Figure 2. Indicate in the figure the initial locations (inside outside of the bag) of all types of molecules available for diffusion through the dialysis membrane. For each of the molecules you list in Figure 2, worst predict under your drawing their net (overall) diffusion direction: in the bag, out of the bag, both in and out of the bag equally, or none ( will not diffuse through the dialysis membrane). Give a reason for each prediction. After 30 minutes, remove the bag from the cup, blot it with a paper towel, and cut a slit in the bag large enough to insert a dropper to obtain a sample for testing. Finally, fill in thelast columns of Table 1. Laboratory drawing: In the drawing of Figure 2, the glucose/starch solution will be inside the dialysis bag, but only the glucose will be able to diffuse through the bag. The starch solution is too large to diffuse through the pores of the dialysis bag. In contrast, glucose is small enough to pass through pores. Likewise, water molecules and IKIs that are outside the dialysis bag will be able to easily enter and exit the pores. For the glucose to reach an easier balance, it will diffuse into the cup. In transosmosis, water molecules move to where there is more concentration to achieve an easier balance. Discussion: My hypothesis of the IKI solution added to the water diffusing into the dialysis bag was correct. Lab A's experiment did not present any conflicts that caused me to revise my predictions. The data tells me that the starch is larger than the pore size of the dialysis tubing, as there was no sign of starch in the dialysis bag. The data also tells me how the glucose solution, IKI, and water molecules are small enough to pass through the pores of the dialysis bag. Conclusion: Depending on how the string was attached to both ends of the dialysis bag, would there be any effect on the amount of IKI or starch that would diffuse into the dialysis bag ? If the string was loose, I think it would allow both the IKI and the starch to diffuse into the bag; However, if the knot was tight, I think it would have the opposite effect. molecules through a semi-permeable membrane? Hypothesis: By adding the different solute concentrations of the solution, I hypothesize that there will be a difference in the solute concentration by osmosis. Objective: The objective is to Observe the amount of solute concentration diffused through a semi-permeable membrane as a function of the molar concentration used in the laboratory. By different molar concentrations you can see the magnitude of the difference.Materials: dialysis tubes, plastic cups, distilled water, beaker, sucrose solutions, paper towels, balance, clipboard, calculator.Procedure: Complete the following steps for each sucrose solution you need to test. Pour 160 to 170 ml of distilled water into a plastic cup and be sure to label the cup with the concentration of sucrose you are going to test. Just like in Lab A, get a piece of dialysis tubing that has been soaked in water and roll the tubing between your thumb and index finger. Close one end of the tube by knotting it or tying it with string. This will form a bag. Using a small funnel, pour 25 ml of sucrose solution into the dialysis bag and smooth the top of the bag by passing it between your thumb and forefinger to expel air. Tie the open end of the bag, but leave enough space in the bag to allow for expansion. Before recording the mass, remember to dry the bag on absorbent paper. Record the initial mass in a table (Table 2). In Table 2, you should have 5 columns (Dialysis Bag Contents, Initial Mass, Final Mass, Mass Change and % Mass Change) and 6 rows (one for each molar concentration). Make sure you give yourself space to write down all your calculations. Immerse the dialysis bag in the cup water and ensure that the part of the bag that contains the sucrose solution is completely covered by the cup water at all times. The next step is to wait 30 minutes before moving on to the next step. After 30 minutes, remove the bag from the cup and dry it on paper towels. Massage the bag and record the final mass in Table 2. Finally,determine the change in mass of the bag and record this data in Table 2. Discussion: The change in mass indicates the water gain of the sucrose solution from the dialysis bag. . The percentage mass change is tested in this experiment. The first variable that could influence the outcome of the experiment is the amount of water outside the dialysis bag because, depending on the amount of water present in the cup, so that both sides of the membrane permeable reach an easier equilibrium, the water molecules will be different. The second variable would be the amount of air in the dialysis bag, because if there is too much air, the water molecules will not be able to diffuse into the dialysis bag. Another variable would be the amount of water absorbed in the string that was attached to the top and bottom of the dialysis bag. The 0.0M bag was found to be hypertonic. If the distilled water were to be put into the dialysis bag, it would become hypertonic as the water would diffuse out of the bag to reach equilibrium. Although there were errors in the experiment that could have impacted the lab, the result we assumed turned out to be correct. My results support my hypothesis because with the amount of concentration in each solution, the mass was either large or small. The 0 M configuration was supposed to be isotonic, but the results turned out to be wrong. If the dialysis was filled with water instead of the sucrose solution, the solution would be hypertonic and the dialysis bag would decrease in mass. Drinking sea water can dehydrate the body since the cell will lose water. Sea water is in greater concentration than the cells that line our small intestine. Therefore, for cells to reach a facilitated balance, the body will lose a lot of water, dehydrating it. Conclusion: Would the amount of concentration in a solution possibly affect the change in mass? I think the different concentration changes the mass of the solution left in the dialysis bag. The more concentration there is in a bag, the more weight it will gain. the more solution, I think the higher the concentration, the more water the potato will lose because it has more water potential. Objective: The objective is to observe and understand water potential by immersing potato cores in sucrose solutions and determining the mass change. We are trying to understand how water will diffuse through living plant tissue (especially potatoes). Materials: Plastic cups, distilled water, sucrose solutions, potato cores (or cork moth, scalpel and potato), plastic wrap, paper towels, scale, thermometer, calculator. Procedure: Label a cup with the concentration of sucrose you will test. Using the corkscrew, cut four cylinders of potato tissue from the potato. Be careful not to stab your hand with the cork borer. Cut the potato cylinders to about 3 cm in length and cut both ends of the cylinder to remove the skin. When cutting the ends, always be careful. Place the potato cylinder in a beaker or cup and cover it with a lid or plastic wrap. Use a scale to determine the total mass of all potato sections. On Table 3, record the initial mass. Your table should have 7 columns (Cup Content, Temperature, Initial Mass, Final Mass, Mass Change, % Mass Change and % Change in Mass Class Average) and 6 rows (one for each molarity) . After placing all the potato sections. into the labeled cup, pour 100 ml of the solution of,.