Various mushrooms widely used in Korea, China and Japan as health stimulants and ancient herbal medicine. A variety of mushrooms have been used traditionally in different cultures for the maintenance of health and in the prevention and treatment of various diseases [1]. The medicinal mushroom is used as a traditional medicine in the Western Ghats of India. Metabolites of the fungus or other related compounds could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms resistant to conventional treatments. medicinal mushrooms and mushrooms contain various bioactive substances, including polysaccharides and proteoglycans, which exert different properties such as antitumor, immunomodulatory, antioxidant, anti-radical, cardiovascular, antihypercholesterolemic, antiviral, antibacterial, antiparasitic, antifungal, detoxifying, hepatoprotective and antidiabetics. effects[2].The antibacterial activity of medicinal mushrooms describes the action of its extracts without identifying the compounds responsible for this activity. The fungi showed antimicrobial activity against pathogenic microorganisms, including bacteria associated with nosocomial infections (Pseudomonas aeruginosa, Pseudomonas maltophila, Listeria monocytogenes, Staphylococcus aureus, Klebsiella pneumoniae, Morganella morganii, Proteus mirabilis, Serratia marcescens, Escherschia coli, Aceinetobacter) and multidrug resistance. In human pathogenic microorganisms, multiple drug resistance has developed due to the indiscriminate use of commercial antimicrobial drugs [3]. Increasing bacterial resistance to antibiotics and antimicrobials is a growing concern facing the medical, food, and health sectors [4]. Among the many fungi, Ph...... middle of paper ......f cork borer (6 mm), and extracts (80 μ/l) were added to each well. The plates were incubated at 35-37°C for 18-24 hours, activity was calculated by measuring the diameter of the inhibition zone and the results were compared and analyzed. Minimum Inhibitory Concentration (MIC) Assay Phellinus MIC test samples were performed by broth dilution method. A set of test tubes containing 9 ml of broth medium was prepared, to the first tube a known concentration of Phellinus sample extract was added and the solution was mixed thoroughly. Serial dilution was performed and after dilution, 10 µl/ml of the microbial test suspension was added to each tube, including the control tubes. The test tubes were incubated at 37°C for 18 to 24 hours. The test tubes were observed for the growth of microorganisms. The concentration of extracts from Phellinus samples was 2 μl/ml to 1024 μl/ml. [5,10].