Thin films of chitosan, produced by solvent casting, were functionalized by incorporating nanoliposomes based on natural plant and marine lecithin. The physicochemical properties were characterized by water contact angle, kinetic swelling test and torsional harmonic atomic force microscopy (TH–AFM) analysis. The surface wettability, swelling ratio, roughness, and local stiffness of chitosan thin films can be modified and controlled by the addition of nanoliposomes. The water contact angle decreases from 75° (±2°) to 50° (±1°) and 38° (±1°) when the amount of soy and salmon nanoliposomes increases in the chitosan films , respectively. the films reach the equilibrium state after seven days in the presence of phosphate-buffered saline. The surface topography of the films is identical in terms of asymmetry and amplitude distribution of roughness measurements but shows a significant increase in asperity height upon incorporation of soy nanoliposomes. This variation induces a reduction in the contact stiffness (0.6 Gpa) and the value of the adhesion force (11.7 nN). One of the main goals is to understand the underlying cell-matrix interactions. To this end, human mesenchymal stem cells (hMSCs) were seeded onto the films. The results of the in vitro biocompatibility analysis showed that the thin films of a nanoliposomes/chitosan mixture are not toxic after 24 hours of cell seeding. The films promote cell adhesion and proliferation when the concentration of soy and salmon nanoliposomes is below 2 mg/mL and 4 mg/mL, respectively. The nanoliposomes/salmon chitosan mixture films particularly showed better cell proliferation in hMSC culture at a concentration of 1 mg/mL. Nanoliposomes functionalized thin films of chitosan at certain concentrations could offer ...... middle of paper ......w surface energy like polymers. The Owens-Wendt theory divides surface energy into two components: one due to dispersive interactions and the other due to polar interactions. 46. ​​Owens and Wendt proved that the total surface energy of a solid can be expressed as the sum of the contributions of the dispersive and polar energies. components of the polar force. These can be determined from the contact angle, θ, data of polar and non-polar liquids with known dispersive and polar parts of their surface energy, via the following relationships: For these measurements, two liquids ( water and diiodomethane) were used. The same Digidrop measuring device was used for surface energy measurement of chitosan and blend films. The total surface energy, polar component and dispersion component of chitosan and nanoliposomes mix thin films previously had been determined from the contact angle of water and diiodomethane..